Description
Product Specifications
COA
IFU
Instrument
Description
Human Chorionic Gonadotropin is a glycoprotein hormone composed of two dissimilar subunits, α and β, joined noncovalently. It is produced by trophoblast tissue in pregnancy and trophoblast disease, and in small amounts by certain poorly differentiated cancers. The α-subunit of hCG is similar to that of the pituitary glycoprotein hormones. It is composed of 92 amino acids linked by five disulfide bridges. The α-subunit has two N-linked oligosaccharide side chains, attached at amino acid residues 52 and 78. The β-subunit is unique, and distinguishes hCG from the other glycoprotein hormones. It is composed of 145 amino acids linked by six disulfide bridges. The b-subunit contains two N-linked oligosaccharide side chains, attached to residues 13 and 30. It also has four O-linked oligosaccharide units, located in the unique proline- and serine-rich C-terminal extension (residues 122 to 145). Serum and urine concentrations of biologically active hCG (nonnicked hCG) rise exponentially in the first trimester of pregnancy, doubling every 48 h, to a peak at about 10 weeks of gestation (weeks since last menstrual period). Concentrations decrease from the 10th to the 16th week of gestation, reaching approximately one-fifth of peak concentrations, and remain around this concentration until term. Determination of hCG is used for diagnosis and monitoring of pregnancy, pregnancy related disorders, for trophoblastic and some nontrophoblastic and some nontrophoblastic tumors. In addition, hCG is determined for doping control in males.
Product Specifications
Intended Use
quantitative determination of Human Chorionic Gonadotropin beta subunit (β-hCG) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The Human Chorionic Gonadotropin beta subunit assay is a quantitative sandwich immunoassay to determine the presence of
βhCG in human serum and plasma using CMIA technology with flexible assay protocols.
- Sample, and paramagnetic anti-βhCG coated microparticles are mixed, βhCG present in the sample binds to anti-βhCG coated microparticles, forming an antigen antibody complex.
- After incubation, a conjugate containing acridinium-labeled anti-βhCG is added to the reaction mixture and binds to unoccupied binding sites of the anti-βhCG coated microparticles.
- After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of βhCG in the sample and the RLUs detected by the optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument