Description
Product Specifications
COA
IFU
Instrument
Description
Cardiac troponin I, often denoted as cTnI, is presented in cardiac muscle tissue by a single isoform with a molecular weight of 23.9 kDa. It consists of 209 amino acid residues. The theoretical pI of cTnI is 9.05. cTnI differs from other troponins due to its N-terminal extension of 26 amino acids. cTnI has been known as a reliable marker of cardiac muscle tissue injury. It is considered to be more sensitive and significantly more specific in diagnosis of the myocardial infarction than the “golden marker” of last decades- CK-MB, as well as total creatine kinase, myoglobin and lactate dehydrogenase isoenzymes. The high-sensitivity cardiac troponin test (hs-cTnI) is the latest generation of the cardiac enzyme testing that allows for detection of very low levels of troponin I, helping to diagnose heart attacks more quickly.
Product Specifications
Intended Use
High Sensitive Cardiac Troponin I Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of Cardiac Troponin I (cTnI) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The High Sensitive Cardiac Troponin I assay is a quantitative sandwich immunoassay to determine the presence of cTnI in human serum and plasma using CMIA technology with flexible assay protocols.
- Sample, paramagnetic anti-cTnI coated microparticles and a conjugate containing acridinium-labeled anti-cTnI are mixed, cTnI present in the sample binds to anti-cTnI coated microparticles and acridinium-labeled anti-cTnI, forming an antigen antibody complex.
- After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of cTnI in the sample and the RLUs detected by the optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument