Description
Product Specifications
COA
IFU
Instrument
Description
Vitamin D (also referred to as “calciferol”) is a fat-soluble vitamin that is naturally present in a few foods, added to others, and available as a dietary supplement. It is also produced endogenously when ultraviolet (UV) rays from sunlight strike the skin and trigger vitamin D synthesis. Vitamin D obtained from sun exposure, foods, and supplements is biologically inert and must undergo two hydroxylations in the body for activation. The first hydroxylation, which occurs in the liver, converts vitamin D to 25-hydroxyvitamin D [25(OH)D], also known as “calcidiol.” The second hydroxylation occurs primarily in the kidney and forms the physiologically active 1,25-dihydroxyvitamin D [1,25(OH)2D], also known as “calcitriol”. Although 1,25(OH)2VD is the active form of vitamin D, Measurement of 25(OH)VD in the serum is a good index for determining vitamin D deficiency and intoxication and aids in diagnosis of patients with metabolic bone diseases due to long half-life and good circulating level. Vitamin D helps the body absorb and use calcium and phosphorus. As a result, vitamin D deficiency causes bone-weakening diseases such as rickets in children and osteoporosis in adults. Recent studies have also established a link between low circulating vitamin D levels and an increased risk of cancer, diabetes and several cardiovascular or autoimmune diseases.
Product Specifications
Intended Use
25-hydroxy Vitamin D Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of 25-hydroxy Vitamin D (25-OH VD) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The 25-hydroxy Vitamin D assay is a quantitative competitive immunoassay to determine the presence of 25-OH VD in human
serum and plasma using CMIA technology with flexible assay protocols.
- Sample, Assay auxiliary 1 and Assay auxiliary 2 are mixed, after incubation, a conjugate containing acridinium-labeled 25-OH VD are mixed, 25-OH VD present in the sample binds to anti-25-OH VD labeled acridinium, forming an antigen antibody complex.
- After incubation, a conjugate containing biotin-labeled anti-25-OH VD and paramagnetic streptavidin coated microparticles are added to the reaction mixture and 25-OH VD binds to unoccupied binding sites of the anti-25-OH VD labeled biotin.
- After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of 25-OH VD in the sample and the RLUs detected by the Optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument