Description
Product Specifications
COA
IFU
Instrument
Description
Cortisol is produced in the human body by the adrenal gland in the zona fasciculata, and the release of cortisol is controlled by the hypothalamus, a part of the brain. The secretion of corticotropin-releasing hormone by the hypothalamus triggers cells in the neighboring anterior pituitary to secrete another hormone, the adreno- corticotropic hormone (ACTH), into the vascular system, through which blood carries it to the adrenal cortex. ACTH stimulates the synthesis of cortisol and other glucocorticoids, mineralocorticoid aldosterone, and dehydroepiandrosterone. Some medical disorders are related to abnormal cortisol production, such as: primary hypercortisolism (Cushing’s syndrome), secondary hypercortisolism (pituitary tumor resulting in Cushing’s disease, pseudo-Cushing’s syndrome), primary hypocortisolism (Addison’s disease, Nelson’s syndrome), secondary hypocortisolism (pituitary tumor, Sheehan’s syndrome).
Product Specifications
Intended Use
Aurora Cortisol Assay is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of Cortisol in human serum and plasma. The assay is intended for in vitro diagnostic use.
Principle
The Aurora Cortisol assay is a quantitative competitive immunoassay to determine the presence of Cortisol in human serum and plasma using CMIA technology with flexible assay protocols.
- Sample, goat anti-rabbit IgG coated microparticles, rabbit anti-human cortisol polyclonal antiserum and acridinium-labeled cortisol are mixed, cortisol present in the sample competes with acridinium-labeled cortisol to bind to the specific polyclonal antiserum.
- After incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of cortisol in the sample and the RLUs detected by the optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument