Description
Product Specifications
COA
IFU
Instrument
Description
Estradiol (E2) is an estrogen steroid hormone and the major female sex hormone. It is also known as 17β-estradiol (to distinguish it from 17α-estradiol) or as estra-1,3,5(10)-triene-3,17β-diol. It has two hydroxyl groups, one at the C3 position and the other at the 17β position, as well as three double bonds in the A ring. Due to its two hydroxyl groups, estradiol is often abbreviated as E2. It is important in the development and maintenance of female reproductive tissues such as the mammary glands, uterus and vagina during puberty, adulthood and pregnancy. It also has important effects in many other tissues including bone, fat, skin, liver, and the brain. Though estradiol levels in males are much lower than in females, estradiol has important roles in males as well. The Estradiol blood test measures the amount of estradiol in the blood. Estradiol levels reflect follicular maturation, the measurement of estradiol is a valuable tool in the assessment of sexual development, amenorrhea etiology, infertility and menopause causes. High levels of estradiol in males are indicative of feminizing syndromes such as gynecomastia.
Product Specifications
Intended Use
Estradiol Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of Estradiol (E2) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The Estradiol assay is a quantitative competitive immunoassay to determine the presence of E2 in human serum and plasma using CMIA technology with flexible assay protocols.
- Sample, and paramagnetic anti-E2 coated microparticles are mixed, E2 present in the sample is displaced from the E2 binding protein and binds to anti-E2 coated microparticles, forming an antigen antibody complex.
- After incubation, a conjugate containing acridinium-labeled E2 is added to the reaction mixture and binds to unoccupied binding sites of the anti-E2 coated microparticles.
- After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of E2 in the sample and the RLUs detected by the Optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument