Description
Product Specifications
COA
IFU
Instrument
Description
Luteinizing hormone (LH) is a glycoprotein hormone composed of two dissimilar subunits, α and β, joined noncovalently. It is produced and released by the group of cells of the anterior pituitary gland. The α-subunit is similar to that of the follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG) and thyroid-stimulating hormone (TSH). Its β-subunit is different from other glycoproteins hormones with specific biochemical characters. The LH level usually varies with the age and sex of an individual. In women, the LH levels vary with the menstrual cycle and menopause. The increased levels of LH can directly affect ovaries. Low levels of LH can lead to secondary ovarian failure due to less secretion of other hormones from the pituitary gland. In men, the hormonal level usually varies with age. The increased levels of LH can show primary and secondary testicular failure. Low levels of LH in adult males lead to decreased sexual desire and sexual dysfunction due to a reduced level of testosterone. For children, the increased levels of LH can cause early onset of puberty in children. The decreased levels of LH in children causes delayed puberty and is an indication of ovarian or testicular failure, hormone deficiency, etc.
Product Specifications
Intended Use
Luteinizing Hormone Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of Luteinizing Hormone (LH) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The Luteinizing Hormone assay is a quantitative sandwich immunoassay to determine the presence of LH in human serum and plasma using CMIA technology with flexible assay protocols.
- Sample, and paramagnetic anti-LH coated microparticles are mixed, LH present in the sample binds to anti-LH coated microparticles, forming an antigen antibody complex.
- After incubation, a conjugate containing acridinium-labeled anti-LH is added to the reaction mixture and binds to unoccupied binding sites of the anti-LH coated microparticles.
- After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of LH in the sample and the RLUs detected by the optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument