Thyroid preoxidase (TPO) is a membrane-bound glycoprotein enzyme with an approximate mass of 103KD. TPO is the key enzyme in the process of thyroid hormone synthesis, which is mainly distributed in the microvilli and endoplasmic reticulum of thyroid follicular cavity. Anti-TPO antibodies are found often in conjunction with anti-Tg in the majority of cases of Hashimoto’s thyroiditis, Primary Myxedema, and Graves’disease. The relationship of autoimmune thyroid disease to pregnancy has been the subject of considerable interest with the recognition of the postpartum thyroid disease syndromes. Anti-TPO antibodies are demonstrable in most cases of postpartum thyroiditis and it has been found that the presence of autoantibody in early pregnancy was associated with a high risk of asymptomatic postpartum hypothyroidism. It is common to find anti-TPO antibodies in the absence of autoantibodies to thyroglobulin, particularly in patients with small goitres and up to 64% of cases of autoimmune hypothyroidism have been reported to be associated with anti-TPO antibodies alone. In addition, anti-TPO antibodies are frequently found in patients with other autoimmune diseases such as Rheumatoid Arthritis, Addison’s Disease and Type I Diabetes. They are also detectable at low levels in up to 20% of asymptomatic individuals,22 particularly the elderly23 and more often in women than in men, although the clinical significance of these autoantibodies is unclear.
Intended Use
Antibody to Thyroid Peroxidase Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative
determination of Antibody to Thyroid Peroxidase (TPOAb) in human serum and plasma. The assay kit is intended for in vitro
diagnostic use.
Principle
The Antibody to Thyroid Peroxidase assay is a quantitative sandwich immunoassay to determine the presence of TPOAb in human serum and plasma using CMIA technology with flexible assay protocols.
- 1.Sample, assay diluent and paramagnetic Biotin-labeled TPO coated microparticles are mixed, TPOAb present in the sample binds to TPO coated microparticles, forming an antigen antibody complex.
- 2.After incubation, a conjugate containing acridinium-labeled Staphylococcal Protein A is added to the reaction mixture and binds to unoccupied binding sites of the TPOAb present in the sample.
- 3.After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of TPOAb in the sample and the RLUs detected by the Optical system. Results are calculated automatically based on the previously established calibration curve
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
