Description
Product Specifications
COA
IFU
Instrument
Description
The thyroid gland mainly secretes two thyroid hormones, one of which is tetraiodide Thyroxine, or thyroxine (T4), accounts for 93%; the other is triiodothyronine (T3), which accounts for 7%; and is 20% in T3 circulating in the blood. T3 circulates in the blood flow as a balanced mixture and a serum-binding hormone. Free T3 (FT3) is an unbound, biologically active form, accounting for only 0.2-0.4% of the total T3. The determination of free T3 has the advantage of being independent of changes in the concentrations and binding properties of binding proteins. Therefore, free T3 values may provide better indication of thyroid dysfunction
Product Specifications
Intended Use
Free Triiodothyronine Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of Free Triiodothyronine (FT3) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The Free Triiodothyronine assay is a quantitative competitive immunoassay to determine the presence of FT3 in human serum and plasma using CMIA technology with flexible assay protocols.
- 1.Sample, and paramagnetic anti-T3 coated microparticles are mixed, T3 present in the sample is displaced from the T3 binding protein and binds to anti-T3 coated microparticles, forming an antigen antibody complex.
- 2.After incubation, a conjugate containing acridinium-labeled T3 is added to the reaction mixture and binds to unoccupied binding sites of the anti-T3 coated microparticles.
- 3.After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- 4.The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of FT3 in the sample and the RLUs detected by the Optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument