Description
Product Specifications
COA
IFU
Instrument
Description
Progesterone (Prog) is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. It belongs to a group of steroid hormones called the progestogens and is the major progestogen in the body. Progesterone has a variety of important functions in the body. It is also a crucial metabolic intermediate in the production of other endogenous steroids, including the sex hormones and the corticosteroids, and plays an important role in brain function as a neurosteroid. The metabolism of progesterone occurs mainly in the liver, though enzymes that metabolize progesterone are also expressed widely in the brain, skin, and various other extrahepatic tissues. In women, progesterone is produced by the adrenal glands (located on the top of the kidneys) as well as the corpus luteum (located in the ovaries). It is also produced by the placenta during pregnancy. Its level relatively low during the preovulatory phase of the menstrual cycle, rise after ovulation, and are elevated during the luteal phase.
Product Specifications
Intended Use
Progesterone Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of Progesterone (Prog) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The Progesterone assay is a quantitative competitive immunoassay to determine the presence of Prog in human serum and plasma using CMIA technology with flexible assay protocols.
- Sample, and paramagnetic anti-Prog coated microparticles are mixed, Prog present in the sample is displaced from the Prog binding protein and binds to anti-Prog coated microparticles, forming an antigen antibody complex.
- After incubation, a conjugate containing acridinium-labeled Prog is added to the reaction mixture and binds to unoccupied binding sites of the anti-Prog coated microparticles.
- After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of Prog in the sample and the RLUs detected by the optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument