Description
Product Specifications
COA
IFU
Instrument
Description
Total Thyroxine (TT4) is an iodine-containing hormone with a molecular weight of approximately 777 daltons secreted by the thyroid gland, which is an indispensable component of the integrity of hypothalamus anterior pituitary thyroid regulatory system. T4 and its associate thyroid hormone T3 are responsible for regulating diverse biochemical processes throughout the body which are essential for normal metabolic and neural activity. T4 is 99.9% bound to serum thyroxine binding proteins (TBP). The hormone is transported bound primarily to thyroxine binding globulin (TBG) and secondarily by thyroxine binding prealbumin (TBPA) and albumin. Less than 0.05% of the total circulating T4 is unbound and therefore biologically active. Clinically, T4 measurements have long been recognized as an aid in the assessment and diagnosis of thyroid status. Elevated T4 values are characteristically seen in patients with overt hyperthyroidism, while T4 levels are generally depressed in patients with overt hypothyroidism. The changes of serum TBG concentration significantly affects the determination result of T4. So, the results of measurement of T4 should also consider the effect of changes in TBG content.
Product Specifications
Intended Use
Total Thyroxine Assay Kit is a chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of Total Thyroxine (TT4) in human serum and plasma. The assay kit is intended for in vitro diagnostic use.
Principle
The Total Thyroxine assay is a quantitative competitive immunoassay to determine the presence of TT4 in human serum and
plasma using CMIA technology with flexible assay protocols.
- Sample, assay auxiliary and paramagnetic anti-T4 coated microparticles are mixed, T4 present in the sample is displaced from the T4 binding protein and binds to anti-T4 coated microparticles, forming an antigen antibody complex.
- After incubation, a conjugate containing acridinium-labeled T4 is added to the reaction mixture and binds to unoccupied binding sites of the anti-T4 coated microparticles.
- After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture.
- The resulting chemiluminescent reaction is measured as relative light units (RLUs). There is a relationship between the amount of TT4 in the sample and the RLUs detected by the optical system. Results are calculated automatically based on the previously established calibration curve.
Storage
The kit should be stored upright, not upside down or horizontally. Store at 2–8°C with a validity period of 12 months.
COA
IFU
Instrument